Fish collagen peptide Usage and extraction method

What is fish collagen peptide? Fish collagen peptide has a LOC elastic net three-dimensional water-locking system that will release a large number of water-locking groups in three dimensions for 12 hours to firmly lock the water supplied in the body to form a "dermal reservoir" to achieve a continuous moisturizing effect; fish collagen peptide repairs broken and aged elastic fiber nets to reorganize the skin tissue structure and stretch wrinkles, which can remove free radicals in the body, resist oxidation and slow down skin aging. Fish collagen peptide is a small molecule oligopeptide with a relative molecular weight of 200~800 Dalton obtained by enzymatic hydrolysis, separation, chemicalbook refining and drying with fish scales or fish skin as raw materials.

 

Fish collagen peptide has a high nutritional value while having physiological regulation functions. Its protein content is more than 90% and contains 19 kinds of amino acids, which is consistent with the needs of people in the world today for nutrition and health. As a small molecule protein peptide, it is easily absorbed and utilized by the human body, will not cause allergic reactions, and can also promote the absorption of vitamins and minerals.

 

Collagen is the most abundant type of protein in the human body, accounting for about 1/4 of the total protein. As an extracellular protein, it exists in human connective tissue, bones and cartilage. It contains more than 90% in bones and tendons, and more than 50% in skin tissue. It is mainly composed of four types of collagen: I, II, III and IV. In organisms, the content of type I, II and III collagen is extremely rich, accounting for 80% to 90% of the total collagen in organisms. The amino acid composition of collagen is different from other proteins. It is rich in glycine, proline and high content of hydroxyproline. Glycine accounts for about 30% of the total amino acids, and the proline content exceeds 10%. Collagen also has good water retention and is an excellent cooperative moisturizer. Collagen products have three functions: protecting skin moisture, increasing bone density and enhancing immunity. They play an important role in beauty, fitness and bone health, and are widely used in functional foods, health products and cosmetics. Fish collagen peptide refers to a small molecule peptide product obtained by proteolysis technology using fish meat or fish skin, fish scales, fish bones and other fish processing byproducts and low-value fish as raw materials.

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Beauty effect

1. Moisturizing: Collagen contains hydrophilic natural moisturizing factors, and the triple helix structure can strongly lock moisture, so that the skin always stays moist and tender!

2.Nourishing: Active collagen has strong permeability to the skin, can penetrate the stratum corneum and combine with skin epithelial cells, participate in and improve the metabolism of
skin cells, and enhance the activity of collagen in the skin.

3. Brightening: The luster of the skin depends on the water content. Collagen peptides have good water retention ability to make the skin moisturized and shiny, exuding a healthy glow.

4. Firming: When collagen is absorbed by the skin, it fills between the dermis of the skin, increases skin density, generates skin tension, shrinks pores, and makes the skin tight and elastic.

5. Anti-wrinkle: The plump collagen layer in the dermis supports the skin cells and combines the effects of moisturizing and inhibiting wrinkles, which together achieves the effect of stretching coarse lines and fading fine lines.

6. Repair: Active collagen can directly penetrate into the bottom layer of the skin, and has good affinity with the surrounding tissues. It can coordinate cells to produce collagen and promote the normal growth of skin cells. At the same time, active collagen itself also has the effects of anti-inflammatory and skin renewal.

7. Breast beauty: The unique hydroxyproline in collagen has the effect of tightening connective tissue, which can make loose tissues firm, support sagging breasts, and make breasts upright, plump and elastic.

Application

Fish collagen peptide is a polypeptide substance that can be used as a nutritional enhancer.

Preparation of fish collagen peptides

Preparation process

A preparation method of fish collagen peptide for beauty and skin care, comprising the following six steps:

S1: Using cod fish bones and fish skin as raw materials, washing the fish bones and fish skin with water, then soaking them in warm water, then rapidly cooling them, freezing and removing acid, taking them out to thaw, washing them with water again, draining them, and mincing them to obtain raw material A;

S2: Adding NaOH solution and ginger solution to raw material A, soaking them for 4h~6h, then washing them with water until the pH value is neutral, centrifuging them, collecting precipitate I, adding NaCl solution to the precipitate, soaking them for 2h~4h, then washing them with water until the pH value is neutral, centrifuging them, collecting precipitate II, adding the mixed solution to the precipitate, and soaking them at 50℃~60℃ for 2h~4h. h, then washed with water until the pH value is neutral, centrifuged, collected the precipitate III, and dried to obtain powder B;

S3: Powder B is suspended in water at a mass ratio of water: powder B = 100: (3~10), and 0.5%~2% bromelain of the mass of powder B is added; reacted in a water bath at 50℃ for 1h~1.5h under the MRM molecular resonance environment with an anion generation amount of 2000 e~-/cm~3~2500 e~-/cm~3, then the enzyme is inactivated, cooled, centrifuged, the supernatant is collected, and spray dried to obtain dry powder C;

S4: At a mass ratio of KCl-HCl buffer: dry powder C = 100: (2~6), dry powder C is suspended in pH1.5, 50mM Add 2% to 4% pepsin by weight of dry powder C to KCl-HCl buffer; react in a water bath at 37°C for 1h to 1.5h, then inactivate the enzyme, cool, centrifuge, collect the supernatant, spray dry, and obtain dry powder D;

S5: Suspend dry powder D in pH 8.0, 50mMTris-HCl buffer at a mass ratio of Tris-HCl buffer: dry powder D = 100: (2 to 6), add 3% to 6% acyltransferase I by weight of dry powder D; react in a water bath at 57°C, 25kHZ ultrasonic reaction for 1h to 1.5h, then inactivate the enzyme; cool, use a 1kDa ultrafiltration membrane for centrifugal filtration rate separation, obtain an ultrafiltrate less than 1kDa, spray dry, and obtain dry powder E;

S6: Suspend dry powder E in the compound liquid at a mass ratio of compound liquid: dry powder E = 100: (20 to 25), mix for 30min to 60min, concentrate, and dry to obtain fish collagen peptide.


Post time: Jan-10-2025